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1.
PeerJ ; 12: e17085, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38618565

RESUMO

Background: Greenhouse vertical farming under natural sunlight is an alternative farming technique that grows crops in a stacking column and extends in a vertical direction. Sunlight availability is one of the crucial factors for crop development in vertical farming. Therefore, this investigation aimed to examine the effect of sunlight availability on lettuce growth and yields at different levels of vertical shelves. Methods: Six shelves were constructed with three levels: upper, middle and lower levels. Lettuces (Lactuca sativa L.) as 'Baby Cos' and 'Green Oak' at 14 days after sowing were planted on the three levels. The photosynthetic photon flux density (PPFD) was recorded, and the PPFD values were then converted to the daily light integral (DLI). Plant height and canopy width were measured three times at 14, 21 and 28 days after transplanting. At maturity, fresh weight (FW) was directly monitored after harvest. Results: The results showed that the highest PPFD and DLI values were found at the upper level (PPFD 697 µmol m-2 s-1 and DLI 29 mol m-2 d-1) in comparison to the middle (PPFD 391 µmol m-2 s-1 and DLI 16 mol m-2 d-1) and lower (PPFD 322 µmol m-2 s-1 and DLI 13 mol m-2 d-1) levels. The lowest plant height and canopy width values were observed on the upper levels for both lettuce varieties during the three measurement dates. The middle ('Baby Cos' = 123.8 g plant-1 and 'Green Oak' = 190.7 g plant-1) and lower ('Baby Cos' = 92.9 g plant-1 and 'Green Oak' = 203.7 g plant-1) levels had the higher values of FW in comparison to the upper level ('Baby Cos' = 84.5 g plant-1 and 'Green Oak' = 97.3 g plant-1). The values of light use efficiency (LUE) showed an increased trend from the upper to lower levels in both varieties, with values of 'Baby Cos' of 0.10 g mol-1 in the upper level, 0.28 g mol-1 in the middle level and 0.26 g mol-1 in the lower level and 'Green Oak' of 0.12 g mol-1 in the upper level, 0.44 g mol-1 in the middle level and 0.57 g mol-1 in the lower level. The findings of the study indicated the viability of utilizing vertical shelves for lettuce production.


Assuntos
Agricultura , Alface , Humanos , Lactente , Tailândia , Fazendas , Produtos Agrícolas
2.
BMC Genomics ; 25(1): 369, 2024 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-38622517

RESUMO

BACKGROUND: Pigeon circovirus infections in pigeons (Columba livia domestica) have been reported worldwide. Pigeons should be PiCV-free when utilized as qualified experimental animals. However, pigeons can be freely purchased as experimental animals without any clear guidelines to follow. Herein, we investigated the status quo of PiCV infections on a pigeon farm in Beijing, China, which provides pigeons for experimental use. RESULTS: PiCV infection was verified in at least three types of tissues in all forty pigeons tested. A total of 29 full-length genomes were obtained and deposited in GenBank. The whole genome sequence comparison among the 29 identified PiCV strains revealed nucleotide homologies of 85.8-100%, and these sequences exhibited nucleotide homologies of 82.7-98.9% as compared with those of the reference sequences. The cap gene displayed genetic diversity, with a wide range of amino acid homologies ranging from 64.5% to 100%. Phylogenetic analysis of the 29 full-genome sequences revealed that the PiCV strains in this study could be further divided into four clades: A (17.2%), B (10.4%), C (37.9%) and D (34.5%). Thirteen recombination events were also detected in 18 out of the 29 PiCV genomes obtained in this study. Phylogenetic research using the rep and cap genes verified the recombination events, which occurred between clades A/F, A/B, C/D, and B/D among the 18 PiCV strains studied. CONCLUSIONS: In conclusion, PiCV infection, which is highly genetically varied, is extremely widespread on pigeon farms in Beijing. These findings indicate that if pigeons are to be used as experimental animals, it is necessary to evaluate the impact of PiCV infection on the results.


Assuntos
Doenças das Aves , Infecções por Circoviridae , Circovirus , Animais , Columbidae , Filogenia , Fazendas , Circovirus/genética , Infecções por Circoviridae/veterinária , Nucleotídeos
3.
Huan Jing Ke Xue ; 45(5): 2913-2925, 2024 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-38629553

RESUMO

In this study, a Meta-analysis was used to investigate the pollution status of eight farmland soil heavy metal elements (As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn) in China. Meanwhile, their spatiotemporal changes and differences between different types of cultivated land were explored. The research data were chosen from 449 relevant literature data collected by CNKI and Web of Science from 2005 to 2021, and the Meta-analysis used a weighted method based on "sampling numbers", "study area", and "standard deviation". The results showed that the national average values of the eight heavy metal elements in Chinese farmland soil were ω(As)11.00 mg·kg-1, ω(Cd)0.350 2 mg·kg-1, ω(Cr)62.91 mg·kg-1, ω(Cu)28.87 mg·kg-1, ω(Hg)0.135 1 mg·kg-1, ω(Ni)28.91 mg·kg-1, ω(Pb)34.67 mg·kg-1,and ω(Zn)90.24 mg·kg-1. Compared with their background values, all elements except As accumulated to some extent, and Cd and Hg accumulated the most, exceeding their background values by 177.9% and 340.3%, respectively. The research results indicated that Cd and Hg were the main pollution elements in farmland soil in China, and their accumulation was mainly influenced by human activities. In terms of their temporal and spatial changes, the Yunnan-Guizhou Plateau and the eastern coast were the most concentrated areas of pollution cases, and the pollution center shifted from the middle reaches of the Yangtze River to the southwest over time. The accumulation of heavy metals in farmland soil was affected by crop planting types, and the accumulation of heavy metals in vegetable and paddy soil was significantly greater than that in other cultivated land types.


Assuntos
Mercúrio , Metais Pesados , Poluentes do Solo , Humanos , Solo , Fazendas , China , Cádmio , Chumbo , Monitoramento Ambiental/métodos , Medição de Risco , Poluentes do Solo/análise , Metais Pesados/análise
4.
Sensors (Basel) ; 24(7)2024 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-38610396

RESUMO

The increasing popularity of pigs has prompted farmers to increase pig production to meet the growing demand. However, while the number of pigs is increasing, that of farm workers has been declining, making it challenging to perform various farm tasks, the most important among them being managing the pigs' health and welfare. This study proposes a pattern mining-based pig behavior analysis system to provide visualized information and behavioral patterns, assisting farmers in effectively monitoring and assessing pigs' health and welfare. The system consists of four modules: (1) data acquisition module for collecting pigs video; (2) detection and tracking module for localizing and uniquely identifying pigs, using tracking information to crop pig images; (3) pig behavior recognition module for recognizing pig behaviors from sequences of cropped images; and (4) pig behavior analysis module for providing visualized information and behavioral patterns to effectively help farmers understand and manage pigs. In the second module, we utilize ByteTrack, which comprises YOLOx as the detector and the BYTE algorithm as the tracker, while MnasNet and LSTM serve as appearance features and temporal information extractors in the third module. The experimental results show that the system achieved a multi-object tracking accuracy of 0.971 for tracking and an F1 score of 0.931 for behavior recognition, while also highlighting the effectiveness of visualization and pattern mining in helping farmers comprehend and manage pigs' health and welfare.


Assuntos
Algoritmos , Reconhecimento Psicológico , Suínos , Animais , Fazendas , Análise de Sistemas
5.
Sensors (Basel) ; 24(7)2024 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-38610405

RESUMO

With the increase in the scale of breeding at modern pastures, the management of dairy cows has become much more challenging, and individual recognition is the key to the implementation of precision farming. Based on the need for low-cost and accurate herd management and for non-stressful and non-invasive individual recognition, we propose a vision-based automatic recognition method for dairy cow ear tags. Firstly, for the detection of cow ear tags, the lightweight Small-YOLOV5s is proposed, and then a differentiable binarization network (DBNet) combined with a convolutional recurrent neural network (CRNN) is used to achieve the recognition of the numbers on ear tags. The experimental results demonstrated notable improvements: Compared to those of YOLOV5s, Small-YOLOV5s enhanced recall by 1.5%, increased the mean average precision by 0.9%, reduced the number of model parameters by 5,447,802, and enhanced the average prediction speed for a single image by 0.5 ms. The final accuracy of the ear tag number recognition was an impressive 92.1%. Moreover, this study introduces two standardized experimental datasets specifically designed for the ear tag detection and recognition of dairy cows. These datasets will be made freely available to researchers in the global dairy cattle community with the intention of fostering intelligent advancements in the breeding industry.


Assuntos
Agricultura , Reconhecimento Psicológico , Animais , Feminino , Bovinos , Fazendas , Indústrias , Inteligência
6.
Sensors (Basel) ; 24(7)2024 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-38610536

RESUMO

Rising platemeters are commonly used in Ireland and New Zealand for managing intensive pastures. To assess the applicability of a commercial rising platemeter operating with a microsonic sensor to estimate herbage mass with its own equation, the objectives were (i) to validate the original equation; (ii) to identify possible factors hampering its accuracy and precision; and (iii) to develop a new equation for heterogeneous swards. A comprehensive dataset (n = 1511) was compiled on the pastures of dairy farms. Compressed sward heights were measured by the rising platemeter. Herbage mass was harvested to determine reference herbage availability. The adequacy of estimating herbage mass was assessed using root mean squared error (RMSE) and mean bias. As the adequacy of the original equation was low, a new equation was developed using multiple regression models. The mean bias and the RMSE for the new equation were overall low with 201 kg dry matter/ha and 34.6%, but it tended to overestimate herbage availability at herbage mass < 500 kg dry matter/ha and underestimate it at >2500 kg dry matter/ha. Still, the newly developed equation for the microsonic sensor-based rising platemeter allows for accurate and precise estimation of available herbage mass on pastures.


Assuntos
Calibragem , Fazendas , Irlanda
7.
Sci Total Environ ; 926: 172115, 2024 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-38569972

RESUMO

Manure composting in traditional small-scale pig farms leads to the migration and diffusion of antibiotics and antibiotics resistance genes (ARGs) along the chain of transmission to the surrounding environment, increasing the risk of environmental resistance. Understanding the transmission patterns, driving factors, and health risks of ARGs on small-scale pig farms is important for effective control of ARGs transmission. This study was conducted on a small pig farm and its surrounding environment. The cross-media transmission of ARGs and their risks in the farming habitat were investigated using Metagenomic annotation and qPCR quantitative detection. The results indicate that ARGs in farms spread with manure pile-soil-channel sediment-mudflat sediment. Pig farm manure contributed 22.49 % of the mudflat sediment ARGs. Mobile genetic elements mediate the spread of ARGs across different media. Among them, tnpA and IS26 have the highest degree. Transmission of high-risk ARGs sul1 and tetM resulted in a 50 % and 116 % increase in host risk for sediment, respectively. This study provides a basis for farm manure management and control of the ARGs spread.


Assuntos
Antibacterianos , Genes Bacterianos , Animais , Suínos , Fazendas , Antibacterianos/farmacologia , Esterco/análise , Resistência Microbiana a Medicamentos/genética , Sequências Repetitivas Dispersas
8.
Science ; 384(6691): 87-93, 2024 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-38574149

RESUMO

Agricultural simplification continues to expand at the expense of more diverse forms of agriculture. This simplification, for example, in the form of intensively managed monocultures, poses a risk to keeping the world within safe and just Earth system boundaries. Here, we estimated how agricultural diversification simultaneously affects social and environmental outcomes. Drawing from 24 studies in 11 countries across 2655 farms, we show how five diversification strategies focusing on livestock, crops, soils, noncrop plantings, and water conservation benefit social (e.g., human well-being, yields, and food security) and environmental (e.g., biodiversity, ecosystem services, and reduced environmental externalities) outcomes. We found that applying multiple diversification strategies creates more positive outcomes than individual management strategies alone. To realize these benefits, well-designed policies are needed to incentivize the adoption of multiple diversification strategies in unison.


Assuntos
Agricultura , Biodiversidade , Conservação dos Recursos Naturais , Ecossistema , Humanos , Fazendas , Solo
9.
BMC Plant Biol ; 24(1): 237, 2024 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-38566021

RESUMO

BACKGROUND: Onions are economically and nutritionally important vegetable crops. Despite advances in technology and acreage, Indian onion growers face challenges in realizing their full productivity potential. This study examines the technical efficiency of onion growers, the factors influencing it, and the constraints faced by those adopting drip irrigation in the Ghod river basin of western Maharashtra. A sample of 480 farmers including those practicing drip irrigation and those not practicing it, was selected from Junnar, Shirur, Parner, and Shrigonda blocks of the basin. The primary data was collected through semi-structured interviews. Analytical tools such as the Cobb-Douglas production function (represents technological relationship between multiple inputs and the resulting output), a single-stage stochastic frontier model, the Tobit model, and descriptive statistics were used to assess the technical efficiency of onion production at the farm level. RESULTS: According to the maximum likelihood estimates of the stochastic frontier analysis, drip adopters exhibited a mean technical efficiency of 92%, while for non-adopters it was 65%. It indicates that the use of drip irrigation technology is associated with higher technical efficiency. The association of technical efficiency and socio-economic characters of households showed that education, extension contacts, social participation, and use of information sources had a positive influence on technical efficiency, while family size had a negative influence on the drip irrigation adopters. For non-drip adopters, significant positive effects were observed for landholding, extension contact, and information source use. The major constraints faced by drip system adopters included a lack of knowledge about the proper operating techniques for drip systems and the cost of maintenance. CONCLUSION: The differences with inputs associated with two irrigation methods showed that the response of inputs to increase onion yield is greater for farmers who use drip irrigation than for farmers who do not, and are a result of the large differences in the technical efficiencies. These inefficiencies and other limitations following the introduction of drip irrigation, such as lack of knowledge about the proper operations, need to be addressed through tailored training for farmers and further interventions.


Assuntos
Irrigação Agrícola , Cebolas , Irrigação Agrícola/métodos , Índia , Fazendas , Produtos Agrícolas
10.
Artigo em Inglês | MEDLINE | ID: mdl-38573743

RESUMO

Facultatively anaerobic bacterial strains were isolated from samples of a methanogenic reactor and, based on 16S rRNA gene sequences, found to be affiliated with the family Propionibacteriaceae in the phylum Actinomycetota. Four strains with almost-identical 16S rRNA gene sequences were comprehensively characterized. The most closely related species to the strains was Brooklawnia cerclae BL-34T (96.4 % sequence similarity). Although most of the phenotypic characteristics of the four strains were identical, distinct differences in some cellular and physiological properties were also detected. Cells of the strains were Gram-stain-positive, non-spore-forming, pleomorphic rods. The strains utilized carbohydrates and organic acids. The strains produced acetate, propionate and lactate from glucose, but the molar ratios of the products were variable depending on the strains. The strains grew at 10-40 °C (optimum at 35 °C) and pH 5.3-8.8 (optimum at pH 6.8-7.5.) The major cellular fatty acids of the strains were anteiso-C15 : 0, C15 : 0 and C15 : 0 dimethylacetal (as a summed feature). The major respiratory quinone was menaquinone MK-9(H4) and the diagnostic diamino acid in the peptidoglycan was meso-diaminopimelic acid. The genome size of the type strain (SH051T) was 3.21 Mb and the genome DNA G+C content was 65.7 mol%. Genes responsible for propionate production through the Wood-Werkman pathway were detected in the genome of strain SH051T. Based on the results of phylogenetic, genomic and phenotypic analyses of the novel strains, the name Brooklawnia propionicigenes sp. nov. is proposed to accommodate the four strains. The type strain of the novel species is SH051T (=NBRC 116195T=DSM 116141T).


Assuntos
Propionatos , Propionibacteriaceae , Bovinos , Animais , Anaerobiose , Fazendas , Filogenia , RNA Ribossômico 16S/genética , Composição de Bases , Ácidos Graxos/química , Análise de Sequência de DNA , DNA Bacteriano/genética , Técnicas de Tipagem Bacteriana , Bactérias Anaeróbias
11.
BMC Vet Res ; 20(1): 126, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38561770

RESUMO

BACKGROUND: Ethno-veterinary practices could be used as a sustainable developmental tool by integrating traditional phytotherapy and husbandry. Phytotherapeutics are available and used worldwide. However, evidence of their antiparasitic efficacy is currently very limited. Parasitic diseases have a considerable effect on pig production, causing economic losses due to high morbidity and mortality. In this respect, especially smallholders and organic producers face severe challenges. Parasites, as disease causing agents, often outcompete other pathogens in such extensive production systems. A total of 720 faecal samples were collected in two farms from three age categories, i.e. weaners, fatteners, and sows. Flotation (Willis and McMaster method), modified Ziehl-Neelsen stained faecal smear, centrifugal sedimentation, modified Blagg technique, and faecal cultures were used to identify parasites and quantify the parasitic load. RESULTS: The examination confirmed the presence of infections with Eimeria spp., Cryptosporidium spp., Balantioides coli (syn. Balantidium coli), Ascaris suum, Oesophagostomum spp., Strongyloides ransomi, and Trichuris suis, distributed based on age category. A dose of 180 mg/kg bw/day of Allium sativum L. and 90 mg/kg bw/day of Artemisia absinthium L. powders, administered for 10 consecutive days, revealed a strong, taxonomy-based antiprotozoal and anthelmintic activity. CONCLUSIONS: The results highlighted the therapeutic potential of both A. sativum and A. absinthium against gastrointestinal parasites in pigs. Their therapeutic effectiveness may be attributed to the content in polyphenols, tocopherols, flavonoids, sterols, sesquiterpene lactones, and sulfoxide. Further research is required to establish the minimal effective dose of both plants against digestive parasites in pigs.


Assuntos
Anti-Infecciosos , Artemisia absinthium , Criptosporidiose , Cryptosporidium , Alho , Enteropatias Parasitárias , Parasitos , Doenças dos Suínos , Animais , Suínos , Feminino , Antiparasitários/farmacologia , Antiparasitários/uso terapêutico , Fazendas , Enteropatias Parasitárias/tratamento farmacológico , Enteropatias Parasitárias/veterinária , Enteropatias Parasitárias/parasitologia , Doenças dos Suínos/tratamento farmacológico , Doenças dos Suínos/parasitologia , Fezes/parasitologia , Prevalência
12.
Arch Virol ; 169(5): 93, 2024 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-38592561

RESUMO

Parsley yellow leaf curl virus (PYLCV) is a new member of the family Geminiviridae that has not yet been assigned to an established genus due to limited information about its biological properties. In this study, the ability of Austroagallia leafhoppers, which are commonly found on vegetable farms in Kerman province (Iran), to transmit this virus was studied. After a two-day acquisition access period, Austroagallia sp. successfully transmitted the virus from PYLCV-infected parsley to healthy seedlings. On the basis of male genitalia morphology, the species of leafhopper was identified as A. sinuata. This is the first report of a transmission of plant virus by a member of the genus Austroagallia.


Assuntos
Geminiviridae , Petroselinum , Fazendas , Geminiviridae/genética , Nível de Saúde , Irã (Geográfico)
13.
Arch Microbiol ; 206(5): 208, 2024 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-38587620

RESUMO

Cistanche deserticola is a precious Chinese medicinal material with extremely high health care and medicinal value. In recent years, the frequent occurrence of stem rot has led to reduced or even no harvests of C. deserticola. The unstandardized use of farm chemicals in the prevention and control processes has resulted in excessive chemical residues, threatening the fragile desert ecological environment. Therefore, it is urgent to explore safe and efficient prevention and control technologies. Biocontrol agents, with the advantages of safety and environment-friendliness, would be an important idea. The isolation, screening and identification of pathogens and antagonistic endophytic bacteria are always the primary basis. In this study, three novel pathogens causing C. deserticola stem rot were isolated, identified and pathogenicity tested, namely Fusarium solani CPF1, F. proliferatum CPF2, and F. oxysporum CPF3. For the first time, the endophytic bacteria in C. deserticola were isolated and identified, of which 37 strains were obtained. Through dual culture assay, evaluation experiment and tissue culture verification, a biocontrol candidate strain Bacillus atrophaeus CE6 with outstanding control effect on the stem rot was screened out. In the tissue culture system, CE6 showed excellent control effect against F. solani and F. oxysporum, with the control efficacies reaching 97.2% and 95.8%, respectively, indicating its great potential for application in the production. This study is of great significance for the biocontrol of plant stem rot and improvement of the yield and quality of C. deserticola.


Assuntos
Cistanche , Bactérias/genética , Meio Ambiente , Fazendas , Caules de Planta
14.
PLoS One ; 19(4): e0299786, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38568879

RESUMO

The feeding strategies of the first domesticated herds had to manage the risks arising from the novelty of livestock practices in territories often distant from the animals' primary habitats. The Iberian Peninsula is characterised by a great diversity of environments, which undoubtedly influenced these dynamics. At the beginning of the Neolithic period these led the possibility to combine diverse livestock farming practices based on different animal feeding habits. This variability is also consistent with the rythms of adoption of domesticated animals, being later on the northern area. In order to address this issue, this work focuses on the dietary regimes of early sheep herds from southern Iberia, an area for which information is currently scarce. This study utilises high-resolution radiocarbon dating and stable isotope data on teeth to investigate sheep husbandry management strategies in Cueva de El Toro (Antequera, Málaga). The radiocarbon dates on the analysed remains evidenced they were deposited at the site over a short period, supporting the recurrent use of the cave. The sequential analysis of oxygen and carbon isotopes in tooth enamel reveals distinct livestock management strategies, reproduction patterns, feeding habits, and mobility during this short period. This variability demonstrates that livestock management practices in the western Mediterranean are more diverse than previously considered. Furthermore, these findings support the hypothesis that early Neolithic communities in the southern Iberian Peninsula were able to adopt different feeding strategies within the same herd, depending on their ecological and productive needs.


Assuntos
Agricultura , Gado , Animais , Ovinos , Isótopos de Carbono , Oxigênio , Fazendas
15.
PLoS One ; 19(4): e0299572, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38568889

RESUMO

The unprecedented impact of the pandemic on both activities and profit of actors draws out the various areas of the value chain that need to be strengthened to ensure resilience in the face of global shock. This study fills the gap by assessing the extent at which COVID-19 impacted the profit of mango value chain actors in southern Ghana. It also analyzed the governance structure and the existing linkages in the dissemination of market information in relation to the profit of the actors. A two-year panel survey on 240 respondents was conducted in 2020 through a multi-stage sampling technique in Greater Accra, Eastern and Volta regions of Ghana. Net Farm Income, Social Network Analysis and Difference-in-Difference models were used in analyzing the data. Findings revealed that mango processors have more bargaining power and make the most profit while producers receive more information than other actors. Farmer-based organizations were found to be the prominent node and influential in the dissemination of market information within the value chain. The outbreak of COVID-19 negatively impacted the profit of mango producers and distributors; however, processors had a positive impact on their profit. The study therefore demonstrated that producers and distributors were vulnerable to the effect of the COVID-19 shock, whilst processors were robust to the shocks. Thus, reformed policies by all stakeholders for emergency preparedness should be targeted especially at those vulnerable actors in the chain. Additionally, FBOs, retailers and other key stakeholders should be considered in policy development to enhance market information dissemination.


Assuntos
COVID-19 , Mangifera , Humanos , COVID-19/epidemiologia , Organizações , Fazendeiros , Fazendas
16.
BMC Plant Biol ; 24(1): 258, 2024 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-38594637

RESUMO

BACKGROUND: Weed control is essential for agricultural floor management in vineyards and the inter-row mulching is an eco-friendly practice to inhibit weed growth via filtering out photosynthetically active radiation. Besides weed suppression, inter-row mulching can influence grapevine growth and the accumulation of metabolites in grape berries. However, the complex interaction of multiple factors in the field challenges the understanding of molecular mechanisms on the regulated metabolites. In the current study, black geotextile inter-row mulch (M) was applied for two vintages (2016-2017) from anthesis to harvest. Metabolomics and transcriptomics analysis were conducted in two vintages, aiming to provide insights into metabolic and molecular responses of Cabernet Sauvignon grapes to M in a semi-arid climate. RESULTS: Upregulation of genes related to photosynthesis and heat shock proteins confirmed that M weakened the total light exposure and grapes suffered heat stress, resulting in lower sugar-acid ratio at harvest. Key genes responsible for enhancements in phenylalanine, glutamine, ornithine, arginine, and C6 alcohol concentrations, and the downward trend in ε-viniferin, anthocyanins, flavonols, terpenes, and norisoprenoids in M grapes were identified. In addition, several modules significantly correlated with the metabolic biomarkers through weighted correlation network analysis, and the potential key transcription factors regulating the above metabolites including VviGATA11, VviHSFA6B, and VviWRKY03 were also identified. CONCLUSION: This study provides a valuable overview of metabolic and transcriptomic responses of M grapes in semi-arid climates, which could facilitate understanding the complex regulatory network of metabolites in response to microclimate changes.


Assuntos
Vitis , Vinho , Vitis/metabolismo , Transcriptoma , Antocianinas/metabolismo , Microclima , Fazendas , Frutas , Vinho/análise
17.
Vet Res ; 55(1): 48, 2024 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-38594744

RESUMO

Actinobacillus pleuropneumoniae (APP) is a bacterium frequently associated with porcine pleuropneumonia. The acute form of the disease is highly contagious and often fatal, resulting in significant economic losses for pig farmers. Serotype diversity and antimicrobial resistance (AMR) of APP strains circulating in north Italian farms from 2015 to 2022 were evaluated retrospectively to investigate APP epidemiology in the area. A total of 572 strains isolated from outbreaks occurring in 337 different swine farms were analysed. The majority of isolates belonged to serotypes 9/11 (39.2%) and 2 (28.1%) and serotype diversity increased during the study period, up to nine different serotypes isolated in 2022. The most common resistances were against tetracycline (53% of isolates) and ampicillin (33%), followed by enrofloxacin, florfenicol and trimethoprim/sulfamethoxazole (23% each). Multidrug resistance (MDR) was common, with a third of isolates showing resistance to more than three antimicrobial classes. Resistance to the different classes and MDR varied significantly depending on the serotype. In particular, the widespread serotype 9/11 was strongly associated with florfenicol and enrofloxacin resistance and showed the highest proportion of MDR isolates. Serotype 5, although less common, showed instead a concerning proportion of trimethoprim/sulfamethoxazole resistance. Our results highlight how the typing of circulating serotypes and the analysis of their antimicrobial susceptibility profile are crucial to effectively manage APP infection and improve antimicrobial stewardship.


Assuntos
Infecções por Actinobacillus , Actinobacillus pleuropneumoniae , Pleuropneumonia , Doenças dos Suínos , Tianfenicol/análogos & derivados , Suínos , Animais , Sorogrupo , Testes de Sensibilidade Microbiana/veterinária , Enrofloxacina , Fazendas , Estudos Retrospectivos , Pleuropneumonia/epidemiologia , Pleuropneumonia/veterinária , Pleuropneumonia/microbiologia , Antibacterianos/farmacologia , Sulfametoxazol/farmacologia , Trimetoprima/farmacologia , Itália/epidemiologia , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/microbiologia , Infecções por Actinobacillus/epidemiologia , Infecções por Actinobacillus/veterinária , Infecções por Actinobacillus/microbiologia , Sorotipagem/veterinária
18.
J Environ Manage ; 357: 120840, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38583381

RESUMO

Agriculture and livestock farming are activities that depend on energy consumption. Photovoltaic self-consumption systems can reduce the production costs of these actors, especially in periods of high-energy price volatility. This work aimed to determine the degree of implementation of photovoltaic self-consumption systems, their relationship with the economic impact of the energy crisis, and the perception of producers to use renewable energy sources in the Spanish agricultural system as it is one of the most important at European level. For this purpose, a survey of the Spanish agricultural and livestock system, involving 396 primary producers, was carried out between December 2022 and March 2023. The results suggest that self-consumption systems are in place in 49.1% of all farms and that these have had a positive effect in alleviating the rising energy costs suffered by the Spanish primary sector. In summary, Spanish primary producers generally have a favorable perception of the use of renewable energies on their farms, especially photovoltaic. However, the cluster analysis shows the fact that there are two types of producers, active and passive, from an environmental point of view. The characterization of this type of producers can help the Spanish Administration to improve the efficiency of its energy strategy, and can be a source of inspiration for the governments of other countries.


Assuntos
Agricultura , Percepção , Agricultura/métodos , Fazendas , Energia Renovável
19.
BMC Public Health ; 24(1): 1096, 2024 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-38643084

RESUMO

BACKGROUND: Pesticide poisoning is a major public health problem in Thailand and is the result of intensive inappropriate and unsafe use of pesticides. This analytical cross-sectional study aimed to determine the factors affecting safe pesticide-use behaviors among farm plant agriculturists in northeastern Thailand. METHODS: The study sample included 427 farm plant agriculturists in Loei Province, northeastern Thailand. Individuals were randomly selected by a multistage random sampling technique. The following data were collected via a self-administered questionnaire consisting of 8 parts: (1) sociodemographic characteristics, (2) knowledge about pesticide use, (3) perceived severity of impact from pesticide use among farm plant agriculturists, (4) perceived susceptibility to pesticide use, (5) perceived self-efficacy in the modification of safe pesticide-use behaviors, (6) perceived outcome of the modification of safe pesticide-use behaviors, (7) social support, and (8) safe pesticide-use behaviors. Frequencies, percentages, means, standard deviations, and multiple regression analyses were employed for data analysis. RESULTS: The majority of participants (79.4%) had high scores for safe pesticide-use behaviors among farm plant agriculturists (scores of 112-150). Factors that significantly affected safe pesticide-use behaviors included knowledge about pesticide use (ß = 0.282), social support (ß = 0.217), reading information from pesticide labels before pesticide use (ß = 0.207), perceived self-efficacy (ß = 0.186), female sex (ß = -0.140), rice farmer status (ß = 0.129), corn farmer status (ß = 0.127), perceived susceptibility (ß = 0.126), having received information from the internet (ß = -0.124), and perceived severity (ß = -0.098). Together, these 10 factors were found to explain 32.5% of the safe pesticide-use behaviors among farm plant agriculturists. CONCLUSIONS: Our findings indicate that there is a need to increase the number of promotional activities related to the safe use of pesticides through social support and training, with the aim of increasing the overall level of knowledge, perceived self-efficacy, perceived susceptibility, and modification of the perceived impact severity of pesticide use. Thus, relevant agencies should promote and support the safe use of pesticides by farm plant agriculturists. This study revealed that the factors affecting safe pesticide-use behaviors among farm plant agriculturists included knowledge about pesticide use, social support, reading pesticide container labels, perceived self-efficacy in the modification of pesticide-use behaviors, sex, rice farmer status, corn farmer status, perceived susceptibility to pesticide use, having received information from the internet, and perceived severity of impact from pesticide use.


Assuntos
Exposição Ocupacional , Praguicidas , Humanos , Fazendas , Agricultura , Tailândia/epidemiologia , Estudos Transversais , Fazendeiros , Zea mays , Conhecimentos, Atitudes e Prática em Saúde
20.
Adv Nutr ; 15(4): 100156, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38616069

RESUMO

Food and nutrition insecurity disproportionately impact low-income households in the United States, contributing to higher rates of chronic diseases among this population. Addressing this challenge is complex because of various factors affecting the availability and accessibility of nutritious food. Short value chain (SVC) models, informally known as local food systems, offer a systemic approach that aims to optimize resources and align values throughout and beyond the food supply chain. Although specific SVC interventions, such as farmers markets, have been studied individually, a comprehensive review of SVC models was pursued to evaluate their relative impact on food security, fruit and vegetable intake, diet quality, health-related markers, and barriers and facilitators to participation among low-income households. Our systematic literature search identified 37 articles representing 34 studies from 2000-2020. Quantitative, qualitative, and mixed-method studies revealed that farmers market interventions had been evaluated more extensively than other SVC models (i.e., produce prescription programs, community-supported agriculture, mobile markets, food hubs, farm stands, and farm-to-school). Fruit and vegetable intake was the most measured outcome; other outcomes were less explored or not measured at all. Qualitative insights highlighted common barriers to SVC use, such as lack of program awareness, limited accessibility, and cultural incongruence, whereas facilitators included health-promoting environments, community cohesion, financial incentives, and high-quality produce. Social marketing and dynamic nutrition education appeared to yield positive program outcomes. Financial incentives were used in many studies, warranting further investigation into optimal amounts across varying environmental contexts. SVC models are increasingly germane to national goals across the agriculture, social, and health care sectors. This review advances the understanding of key knowledge gaps related to their implementation and impact; it emphasizes the need for research to analyze SVC potential comprehensively across the rural-urban continuum and among diverse communities through long-term studies of measurable health impact and mixed-method studies investigating implementation best practices. This trial was registered at PROSPERO as CRD42020206532.


Assuntos
Frutas , Estado Nutricional , Humanos , Pobreza , Agricultura , Fazendas
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